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Introduction:

MicroRNAs (miRNAs) are small non-coding RNA molecules that play a crucial role in the post-transcriptional regulation of gene expression. These small RNA molecules have been found to be involved in various biological processes such as cell proliferation, differentiation, apoptosis, and development. In recent years, there has been a growing interest in understanding the role of miRNAs in gene expression, as dysregulation of miRNAs has been linked to various diseases including cancer, cardiovascular diseases, and neurological disorders. This thesis aims to explore the role of miRNAs in gene expression and its implications in human health and disease.

Table of Content:

1.1 Introduction
1.2 Background of study
1.3 Problem Statement
1.4 Objective of study
1.5 Limitation of study
1.6 Scope of study
1.7 Significance of study
1.8 Structure of the Thesis
1.9 Definition of terms

Chapter two: Literature Review
2.1 Overview of microRNAs
2.2 Biogenesis of microRNAs
2.3 Mechanism of microRNA-mediated gene regulation
2.4 Role of microRNAs in development
2.5 Dysregulation of microRNAs in diseases
2.6 Diagnostic and therapeutic potential of microRNAs
2.7 MicroRNAs as biomarkers
2.8 Regulatory networks of microRNAs
2.9 Computational approaches for miRNA target prediction
2.10 Future perspectives in miRNA research

Chapter three: Research Methodology
3.1 Selection of miRNAs for study
3.2 Experimental techniques for miRNA analysis
3.3 Data collection and analysis
3.4 Bioinformatics tools for miRNA target prediction
3.5 Cell culture and transfection experiments
3.6 Animal models for miRNA research
3.7 Statistical analysis
3.8 Ethical considerations in miRNA research

Chapter four: Discussion of Findings
4.1 Expression patterns of miRNAs in different tissues
4.2 Functional analysis of miRNAs in gene regulation
4.3 Identification of miRNA targets
4.4 Validation of miRNA-mediated gene regulation
4.5 Signaling pathways regulated by miRNAs
4.6 Comparison with previous studies
4.7 Implications of study findings
4.8 Future directions in miRNA research

Chapter five: Conclusion and Summary
5.1 Summary of key findings
5.2 Contributions to the field of miRNA research
5.3 Limitations of the study
5.4 Recommendations for future research
5.5 Conclusion

Thesis Overview:

The role of microRNAs (miRNAs) in gene expression has garnered significant attention in recent years due to their involvement in various biological processes and diseases. This thesis aims to provide a comprehensive understanding of the mechanisms by which miRNAs regulate gene expression and their implications in human health and disease. The literature review will cover the biogenesis of miRNAs, their mechanism of action, and their role in development and disease. The research methodology will detail the selection of miRNAs for study, experimental techniques, and data analysis. The discussion of findings will focus on the expression patterns of miRNAs, their functional analysis, and identification of target genes. The conclusion will summarize the key findings, contributions to the field, limitations, and recommendations for future research. This thesis will contribute to the growing body of knowledge on the role of miRNAs in gene expression and their potential therapeutic applications.

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