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Introduction:

RNA interference (RNAi) mechanisms have revolutionized the field of molecular biology by providing a powerful tool for gene silencing. This process involves the post-transcriptional regulation of gene expression by small RNA molecules, leading to the degradation or inhibition of target mRNAs. RNAi mechanisms have been widely studied and utilized in various research areas, including genetic engineering, functional genomics, and therapeutic applications. Understanding the intricacies of RNAi mechanisms is crucial for advancing our knowledge of gene regulation and developing new therapeutic strategies for treating genetic diseases.

Table of Contents:

Chapter 1: Introduction
1.1 Introduction
1.2 Background of Study
1.3 Problem Statement
1.4 Objective of Study
1.5 Limitation of Study
1.6 Scope of Study
1.7 Significance of Study
1.8 Structure of the Thesis
1.9 Definition of Terms

Chapter 2: Literature Review
2.1 Historical Overview of RNA Interference
2.2 Mechanisms of RNA Interference
2.3 Small Interfering RNA (siRNA)
2.4 MicroRNA (miRNA)
2.5 Applications of RNA Interference
2.6 RNA Interference in Gene Therapy
2.7 Challenges and Limitations of RNA Interference
2.8 Recent Advances in RNA Interference Research
2.9 Future Perspectives of RNA Interference
2.10 Summary of Literature Review

Chapter 3: Research Methodology
3.1 Research Design
3.2 Sample Selection
3.3 Data Collection Methods
3.4 Data Analysis Techniques
3.5 Ethical Considerations
3.6 Validity and Reliability of Data
3.7 Research Limitations
3.8 Research Timeline

Chapter 4: Discussion of Findings
4.1 Analysis of Research Data
4.2 Interpretation of Results
4.3 Comparison with Existing Literature
4.4 Implications of Findings
4.5 Recommendations for Future Research
4.6 Conclusion

Chapter 5: Conclusion and Summary
5.1 Summary of Findings
5.2 Contributions to Knowledge
5.3 Practical Implications
5.4 Limitations of the Study
5.5 Directions for Future Research
5.6 Conclusion

Thesis Overview:

RNA interference (RNAi) mechanisms play a crucial role in gene silencing by regulating gene expression at the post-transcriptional level. This thesis aims to provide a comprehensive overview of RNAi mechanisms, including the historical background, molecular pathways, and applications in gene therapy. The literature review will cover key concepts in RNAi research, challenges and limitations, recent advances, and future perspectives. The research methodology section will detail the design, data collection methods, and analysis techniques used in the study. The discussion of findings will analyze and interpret the research data, compare with existing literature, and provide recommendations for future research. The conclusion and summary section will summarize the key findings, contributions to knowledge, practical implications, limitations, and directions for future research in RNA interference mechanisms in gene silencing.

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