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Introduction:
Circular RNAs (circRNAs) have emerged as a novel class of non-coding RNAs that play crucial roles in various cellular processes. These closed-loop structures are generated through back-splicing of exons, leading to a covalently closed loop devoid of 5′ to 3′ polarity and polyadenylated tails. CircRNAs have been implicated in regulating gene expression at transcriptional and post-transcriptional levels, thereby influencing various biological processes such as cell proliferation, differentiation, and apoptosis.
Pharmacological modulation of circRNAs presents a promising avenue for therapeutic intervention in various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases. By targeting circRNAs, researchers can potentially alter gene expression patterns and signaling pathways to treat or prevent disease progression. This thesis aims to provide a comprehensive overview of the current understanding of circRNAs and their pharmacological modulation strategies.
Table of Contents:
Chapter 1: Introduction
1.1 Introduction
1.2 Background of Study
1.3 Problem Statement
1.4 Objective of Study
1.5 Limitation of Study
1.6 Scope of Study
1.7 Significance of Study
1.8 Structure of the Thesis
1.9 Definition of Terms
Chapter 2: Literature Review
2.1 Overview of circRNAs
2.2 Biogenesis and functions of circRNAs
2.3 Role of circRNAs in disease pathogenesis
2.4 Pharmacological modulation of circRNAs
2.5 Techniques for studying circRNAs
2.6 Current challenges in circRNA research
2.7 Therapeutic potential of circRNAs
2.8 Clinical applications of circRNA modulation
2.9 Regulatory considerations for circRNA-based therapeutics
2.10 Future directions in circRNA research
Chapter 3: Research Methodology
3.1 Study design
3.2 Sample collection and processing
3.3 RNA extraction and quantification
3.4 CircRNA profiling techniques
3.5 In vitro and in vivo circRNA modulation assays
3.6 Data analysis and interpretation
3.7 Statistical analysis
3.8 Ethical considerations
Chapter 4: Discussion of Findings
4.1 CircRNAs as potential therapeutic targets
4.2 Pharmacological approaches to circRNA modulation
4.3 Challenges and opportunities in circRNA-based therapeutics
4.4 Clinical implications of circRNA modulation
4.5 Comparison with other RNA-based therapeutics
4.6 Safety and toxicity considerations
4.7 Future perspectives in circRNA research
Chapter 5: Conclusion and Summary
5.1 Summary of key findings
5.2 Implications for future research
5.3 Conclusion
5.4 Recommendations for further studies
5.5 Overall significance of the study
Thesis Overview:
Pharmacological modulation of circular RNAs (circRNAs) has emerged as a cutting-edge research area with immense potential for therapeutic intervention in various diseases. This thesis aims to provide a comprehensive overview of the current understanding of circRNAs and their pharmacological modulation strategies.
Chapter 1 provides an introduction to circRNAs, outlining their biogenesis, functions, and roles in disease pathogenesis. The chapter also sets the stage for the study by presenting the problem statement, research objectives, scope, and significance of the research.
Chapter 2 delves into a detailed literature review on circRNAs, covering topics such as their biogenesis, functions, disease associations, pharmacological modulation strategies, and potential clinical applications. The chapter also discusses current challenges and future directions in circRNA research.
Chapter 3 focuses on the research methodology employed in the study, detailing study design, sample collection and processing, circRNA profiling techniques, data analysis, and ethical considerations. This chapter provides insights into the experimental approach used to investigate circRNA modulation.
Chapter 4 presents a thorough discussion of the study findings, highlighting the potential of circRNAs as therapeutic targets, pharmacological approaches to circRNA modulation, challenges and opportunities in circRNA-based therapeutics, as well as clinical implications and safety considerations.
Chapter 5 concludes the thesis by summarizing key findings, discussing implications for future research, providing recommendations for further studies, and emphasizing the overall significance of the study in advancing the field of pharmacological modulation of circRNAs. This thesis aims to contribute to the growing body of knowledge on circRNAs and their therapeutic potential in disease intervention.
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