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Introduction:

RNA interference (RNAi) is a powerful mechanism that regulates gene expression and plays a crucial role in various biological processes such as development, immunity, and stress responses. It involves the silencing of specific genes by small RNA molecules, thereby controlling the production of proteins encoded by these genes. Understanding the mechanisms of RNA interference is essential for developing novel therapeutic strategies for various diseases, including cancer, viral infections, and genetic disorders.

Chapter 1: Introduction
1.1 Introduction
1.2 Background of study
1.3 Problem Statement
1.4 Objective of study
1.5 Limitation of study
1.6 Scope of study
1.7 Significance of study
1.8 Structure of the Thesis
1.9 Definition of terms

Chapter 2: Literature Review
2.1 Historical overview of RNA interference
2.2 Mechanisms of RNA interference
2.3 Small interfering RNA (siRNA)
2.4 MicroRNA (miRNA)
2.5 RNA-induced gene silencing complex (RISC)
2.6 Off-target effects of RNA interference
2.7 Therapeutic applications of RNA interference
2.8 Challenges and limitations of RNA interference
2.9 Future perspectives of RNA interference
2.10 Recent advancements in the field of RNA interference

Chapter 3: Research Methodology
3.1 Research design
3.2 Data collection methods
3.3 Sample selection
3.4 Data analysis techniques
3.5 Ethical considerations
3.6 Validity and reliability of the study
3.7 Research limitations
3.8 Future research directions

Chapter 4: Discussion of Findings
4.1 Overview of the study results
4.2 Analysis of the data
4.3 Comparison with existing literature
4.4 Implications of the findings
4.5 Recommendations for future research
4.6 Practical implications of the study
4.7 Limitations of the study
4.8 Conclusion
4.9 Contributions to the field
4.10 Suggestions for further research

Chapter 5: Conclusion and Summary
5.1 Summary of the study
5.2 Key findings
5.3 Implications for practice
5.4 Recommendations for policymakers
5.5 Conclusion and final thoughts

Thesis Overview:

The mechanisms of RNA interference represent a cutting-edge field in molecular biology with significant implications for the treatment of various diseases. This thesis aims to provide a comprehensive overview of RNA interference, including its historical background, mechanisms of action, therapeutic applications, and future perspectives. By conducting a thorough literature review and research methodology, this study will explore the challenges and limitations of RNA interference, as well as its potential for revolutionizing the field of gene regulation. The discussion of findings will analyze the data collected and offer recommendations for future research, while the conclusion will summarize the key findings and suggest practical implications for the field. Ultimately, this thesis seeks to contribute to the understanding of RNA interference and its role in shaping the future of biomedical research.

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