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Introduction

Cellular senescence is a complex biological process characterized by irreversible growth arrest, altered gene expression, and the secretion of pro-inflammatory factors. It plays a crucial role in aging, tumor suppression, and tissue regeneration. Despite its significance, the mechanisms underlying cellular senescence are not fully understood. This thesis aims to delve into the various pathways and regulators involved in cellular senescence, shedding light on the intricate network of signaling cascades that govern this phenomenon.

Chapter 1: Introduction

1.1 Introduction
1.2 Background of study
1.3 Problem Statement
1.4 Objective of study
1.5 Limitation of study
1.6 Scope of study
1.7 Significance of study
1.8 Structure of the Thesis
1.9 Definition of terms

Chapter 2: Literature Review

2.1 Historical perspectives on cellular senescence
2.2 Molecular pathways of cellular senescence
2.3 Role of telomere shortening in senescence
2.4 Senescence-associated secretory phenotype (SASP)
2.5 Senescence in aging and age-related diseases
2.6 Senescence in tumor suppression
2.7 Therapeutic targeting of senescent cells
2.8 Epigenetic regulation of senescence
2.9 Crosstalk between senescence and other cellular processes
2.10 Future directions in senescence research

Chapter 3: Research Methodology

3.1 Cell culture techniques
3.2 Senescence induction assays
3.3 Gene expression analysis
3.4 Protein expression analysis
3.5 Immunofluorescence microscopy
3.6 Flow cytometry
3.7 Animal models of senescence
3.8 Bioinformatic analysis of senescence-related pathways

Chapter 4: Discussion of Findings

4.1 Characterization of senescent cells
4.2 Signaling pathways regulating senescence
4.3 Role of senescence in aging and age-related diseases
4.4 Therapeutic implications of targeting senescent cells
4.5 Mechanistic insights into senescence-associated phenotypes
4.6 Epigenetic modifications in senescent cells
4.7 Future perspectives on senescence research

Chapter 5: Conclusion and Summary

The thesis will conclude with a summary of the key findings and a discussion of their implications for understanding the mechanisms of cellular senescence. Future directions for research in this field will also be highlighted.

Thesis Overview

The process of cellular senescence is a fundamental aspect of aging and age-related diseases. This thesis aims to provide a comprehensive understanding of the mechanisms underlying cellular senescence, with a focus on the molecular pathways, regulators, and functional consequences of senescent cells. Through a detailed literature review, research methodology, discussion of findings, and conclusion, this thesis will contribute to the growing body of knowledge on cellular senescence and its implications for aging and disease.

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