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Introduction

Marine ecosystems are among the most diverse and complex environments on Earth, home to a wide variety of species that play crucial roles in maintaining the balance of these ecosystems. Understanding the distribution and diversity of marine species is essential for effective conservation and management strategies. Traditional methods for studying marine biodiversity, such as visual surveys and tissue sampling, are often time-consuming, labor-intensive, and may not provide a comprehensive picture of species distribution.

Environmental DNA (eDNA) techniques have emerged as a promising tool for studying marine biodiversity. eDNA refers to the genetic material that organisms shed into their surrounding environment, such as water or sediment. By analyzing eDNA samples, researchers can detect the presence of species in a non-invasive and cost-effective manner, providing valuable insights into species distribution and diversity.

This thesis aims to explore the use of eDNA techniques for studying the distribution and diversity of marine species. The research will focus on how eDNA can be used to complement traditional methods and enhance our understanding of marine ecosystems. By comparing the results of eDNA analysis with traditional survey data, the study aims to evaluate the reliability and accuracy of eDNA techniques for marine biodiversity research.

Chapter 1: Introduction
1.1 Introduction
1.2 Background of study
1.3 Problem Statement
1.4 Objective of study
1.5 Limitation of study
1.6 Scope of study
1.7 Significance of study
1.8 Structure of the Thesis
1.9 Definition of terms

Chapter 2: Literature Review
2.1 Overview of marine biodiversity
2.2 Traditional methods for studying marine biodiversity
2.3 Introduction to environmental DNA (eDNA)
2.4 Application of eDNA in marine biodiversity research
2.5 Comparison of eDNA techniques with traditional methods
2.6 Challenges and limitations of eDNA techniques
2.7 Advances in eDNA technology
2.8 Case studies of eDNA applications in marine biodiversity research
2.9 Current gaps in knowledge
2.10 Future directions for eDNA research in marine biodiversity

Chapter 3: Research Methodology
3.1 Study area and sample collection
3.2 eDNA extraction and analysis
3.3 Data analysis and interpretation
3.4 Quality control and validation
3.5 Comparison with traditional survey data
3.6 Ethical considerations
3.7 Data management
3.8 Statistical analysis

Chapter 4: Discussion of Findings
4.1 Comparison of eDNA results with traditional survey data
4.2 Reliability and accuracy of eDNA techniques
4.3 Implications for marine biodiversity research
4.4 Strengths and limitations of the study
4.5 Recommendations for future research
4.6 Management and conservation implications
4.7 Policy recommendations
4.8 Conclusion

Chapter 5: Conclusion and Summary
5.1 Summary of key findings
5.2 Contributions to the field
5.3 Implications for conservation and management
5.4 Limitations of the study
5.5 Future research directions
5.6 Conclusion

Thesis Overview:

Exploring the use of environmental DNA (eDNA) techniques for studying the distribution and diversity of marine species is a critical research topic in the field of marine biodiversity. This thesis aims to evaluate the applicability of eDNA techniques in studying marine species and their use in complementing traditional methods. The research will involve a comprehensive literature review, research methodology focusing on eDNA extraction and analysis, comparison of eDNA results with traditional survey data, and a detailed discussion of findings. The thesis will provide valuable insights into the reliability, accuracy, and potential of eDNA techniques for marine biodiversity research, with implications for conservation and management strategies.

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