Exploring the role of extracellular vesicles in cell-to-cell communication and their therapeutic applications – Complete Phd and Masters Thesis

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Introduction

Cell-to-cell communication is essential for the proper functioning of multicellular organisms. While traditional methods of communication between cells have been well studied, recent research has highlighted the role of extracellular vesicles in mediating intercellular communication. Extracellular vesicles are small membrane-bound particles released by cells that contain a diverse array of biomolecules, including proteins, nucleic acids, and lipids. These vesicles play a crucial role in transferring biological information between cells and are involved in processes such as immune response regulation, tissue repair, and cancer progression.

This thesis aims to explore the role of extracellular vesicles in cell-to-cell communication and their therapeutic applications. By understanding the mechanisms by which extracellular vesicles mediate communication between cells, we can potentially develop novel therapeutic strategies for a wide range of diseases. Additionally, this research will contribute to our knowledge of the complex networks of intercellular communication that are essential for maintaining homeostasis in the body.

1.1 Introduction
1.2 Background of study
1.3 Problem Statement
1.4 Objective of study
1.5 Limitation of study
1.6 Scope of study
1.7 Significance of study
1.8 Structure of the Thesis
1.9 Definition of terms

Chapter Two: Literature Review
2.1 Overview of extracellular vesicles
2.2 Biogenesis of extracellular vesicles
2.3 Cargo of extracellular vesicles
2.4 Role of extracellular vesicles in immune regulation
2.5 Role of extracellular vesicles in cancer progression
2.6 Therapeutic applications of extracellular vesicles
2.7 Isolation and characterization of extracellular vesicles
2.8 Challenges and limitations in studying extracellular vesicles
2.9 Current research trends in the field
2.10 Gaps in the literature

Chapter Three: Research Methodology
3.1 Research design
3.2 Sample collection and processing
3.3 Isolation and characterization of extracellular vesicles
3.4 Cell culture experiments
3.5 In vivo studies
3.6 Data analysis
3.7 Ethical considerations
3.8 Timeline

Chapter Four: Discussion of Findings
4.1 Characterization of extracellular vesicles
4.2 Role of extracellular vesicles in cell-to-cell communication
4.3 Therapeutic potential of extracellular vesicles
4.4 Comparison with other methods of intercellular communication
4.5 Future directions for research
4.6 Implications for clinical practice
4.7 Limitations of the study
4.8 Recommendations for further research

Chapter Five: Conclusion and Summary
5.1 Summary of findings
5.2 Contributions to the field
5.3 Implications for future research
5.4 Concluding remarks

Thesis Overview:

Extracellular vesicles (EVs) are gaining increasing attention in the field of cell-to-cell communication and their therapeutic applications. This thesis aims to explore the role of EVs in mediating intercellular communication and their potential use in therapeutic interventions. Chapter One provides an introduction to the topic, including the background of the study, problem statement, objectives, limitations, scope, significance, structure of the thesis, and definitions of key terms.

Chapter Two presents a comprehensive literature review, covering topics such as EV biogenesis, cargo content, immune regulation, cancer progression, and therapeutic applications. Chapter Three outlines the research methodology, including sample collection, EV isolation and characterization, cell culture experiments, in vivo studies, data analysis, ethical considerations, and timeline.

Chapter Four delves into the discussion of findings, including the characterization of EVs, their role in cell-to-cell communication, therapeutic potential, comparison with other communication methods, future research directions, implications for clinical practice, limitations, and recommendations for further research. Finally, Chapter Five provides a conclusion and summary of the thesis findings, contributions to the field, implications for future research, and concluding remarks. Through this comprehensive study, we aim to advance our understanding of EVs in cell communication and their therapeutic applications.

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