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Table of Contents:

Chapter 1: Introduction
1.1 Background of the Study
1.2 Research Problem
1.3 Research Questions
1.4 Objectives of the Study
1.5 Significance of the Study
1.6 Limitations of the Study
1.7 Scope of the Study

Chapter 2: Literature Review
2.1 Introduction to Enzyme Engineering
2.2 Regio-selectivity in Enzymes
2.3 Methods for Improving Regio-selectivity
2.4 Previous Studies on Enzyme Engineering for Regio-selectivity

Chapter 3: Research Methodology
3.1 Research Design
3.2 Data Collection Methods
3.3 Data Analysis Techniques
3.4 Ethical Considerations
3.5 Limitations of the Research Methodology

Chapter 4: Discussion of Findings
4.1 Analysis of Experimental Results
4.2 Comparison with Previous Studies
4.3 Implications of Findings
4.4 Suggestions for Future Research

Chapter 5: Conclusion and Summary
5.1 Summary of Findings
5.2 Conclusion
5.3 Recommendations for Practice
5.4 Recommendations for Future Research

Brief Overview:

Enzymes play a crucial role in various biochemical reactions, and their regio-selectivity – the ability to perform specific reactions at particular locations within a molecule – is of great importance in the field of enzyme engineering. The engineering of enzymes for improved regio-selectivity has been a topic of interest for researchers, as it can lead to the development of more efficient and sustainable biocatalysts for industrial applications.

In this study, the aim is to investigate methods for enhancing regio-selectivity in enzymes through protein engineering techniques. By modifying the active site or substrate binding pocket of enzymes, researchers can tailor their specificity towards target substrates and reactions. The literature review will explore previous studies on enzyme engineering for regio-selectivity, while the research methodology will detail the experimental design and data analysis techniques.

The discussion of findings will analyze experimental results and compare them with previous studies, highlighting the implications of the research for future studies in the field. The conclusion and summary will provide a concise overview of the key findings, recommendations for practice, and suggestions for further research in the area of enzyme engineering for improved regio-selectivity.

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