Developing a protocol for the extraction and analysis of DNA from fingernail clippings – Complete Phd and Masters Thesis

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Introduction:

In recent years, the field of forensic science has seen a significant advancement in DNA analysis techniques, leading to a higher success rate in identifying individuals based on their unique genetic profiles. Fingernail clippings, often overlooked as a potential source of DNA, have been found to contain valuable genetic information that could aid in criminal investigations or the identification of victims in disaster scenarios. However, the extraction and analysis of DNA from fingernail clippings present unique challenges that require the development of a specific protocol.

Chapter One: Introduction
1.1 Introduction
1.2 Background of study
1.3 Problem Statement
1.4 Objective of study
1.5 Limitation of study
1.6 Scope of study
1.7 Significance of study
1.8 Structure of the Thesis
1.9 Definition of Terms

Chapter Two: Literature Review
2.1 Importance of DNA analysis in forensic science
2.2 Previous studies on DNA extraction from fingernail clippings
2.3 Techniques for DNA extraction and analysis
2.4 Challenges in DNA extraction from fingernail clippings
2.5 Advances in forensic DNA analysis
2.6 Legal considerations in forensic DNA analysis
2.7 Applications of DNA analysis in criminal investigations
2.8 Ethical considerations in DNA analysis
2.9 Future trends in forensic DNA analysis
2.10 Summary of Literature Review

Chapter Three: Research Methodology
3.1 Research design
3.2 Sampling method
3.3 Data collection procedures
3.4 DNA extraction protocol development
3.5 DNA analysis techniques
3.6 Validation of DNA extraction protocol
3.7 Statistical analysis
3.8 Quality control measures

Chapter Four: Discussion of Findings
4.1 Evaluation of DNA extraction protocol
4.2 Comparison with existing DNA extraction methods
4.3 Interpretation of results
4.4 Implications for forensic science
4.5 Recommendations for further research

Chapter Five: Conclusion and Summary
5.1 Summary of findings
5.2 Conclusions
5.3 Contributions to the field
5.4 Limitations of the study
5.5 Recommendations for future research

Thesis Overview:

The field of forensic science has seen significant advancements in DNA analysis techniques, leading to higher success rates in identifying individuals based on their unique genetic profiles. Fingernail clippings have been recognized as a valuable source of DNA, but the extraction and analysis of DNA from this source present unique challenges. This thesis aims to develop a protocol for the extraction and analysis of DNA from fingernail clippings to aid in criminal investigations and disaster victim identification.

The thesis will begin with an introduction that provides the background of the study, outlines the problem statement, and sets the objectives, limitations, scope, and significance of the study. The structure of the thesis and key definitions will also be provided in this chapter.

The literature review chapter will explore the importance of DNA analysis in forensic science, previous studies on DNA extraction from fingernail clippings, techniques for DNA extraction and analysis, challenges, advances, legal and ethical considerations, and future trends in forensic DNA analysis.

The research methodology chapter will outline the research design, sampling method, data collection procedures, development of the DNA extraction protocol, DNA analysis techniques, validation of the protocol, statistical analysis, and quality control measures.

The discussion of findings chapter will evaluate the developed DNA extraction protocol, compare it with existing methods, interpret the results, discuss implications for forensic science, and make recommendations for further research.

The conclusion and summary chapter will summarize the findings, draw conclusions, discuss contributions to the field, acknowledge limitations, and provide recommendations for future research in the area of DNA extraction and analysis from fingernail clippings.

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