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Introduction

Cellular and molecular pharmacology of G-protein coupled receptors (GPCRs) is a complex and fascinating area of study in the field of pharmacology. GPCRs are a large family of cell surface receptors that play crucial roles in mediating cellular responses to a wide range of stimuli, including hormones, neurotransmitters, and sensory signals. Due to their involvement in numerous physiological processes, GPCRs are important targets for drug development and therapy.

This thesis aims to provide a comprehensive overview of the cellular and molecular pharmacology of GPCRs, focusing on the mechanisms by which these receptors transduce extracellular signals into intracellular responses. The study will also explore the implications of GPCR signaling in various diseases and the development of novel therapeutic strategies targeting GPCRs.

Chapter 1: Introduction
1.1 Introduction
1.2 Background of study
1.3 Problem Statement
1.4 Objective of study
1.5 Limitation of study
1.6 Scope of study
1.7 Significance of study
1.8 Structure of the Thesis
1.9 Definition of terms

Chapter 2: Literature Review
2.1 Overview of GPCRs
2.2 Molecular structure of GPCRs
2.3 GPCR signaling pathways
2.4 Role of GPCRs in disease
2.5 Pharmacological targeting of GPCRs
2.6 Novel drug discovery strategies targeting GPCRs
2.7 GPCR biased signaling
2.8 GPCR allosteric modulation
2.9 GPCR desensitization and internalization
2.10 GPCR signaling in cancer

Chapter 3: Research Methodology
3.1 Study design
3.2 Selection of GPCR targets
3.3 Cell culture and transfection techniques
3.4 GPCR expression and purification
3.5 Ligand binding assays
3.6 Functional assays for GPCR signaling
3.7 High-throughput screening methods
3.8 Computational modeling and virtual screening

Chapter 4: Discussion of Findings
4.1 Molecular mechanisms of GPCR activation
4.2 Role of GPCRs in cardiovascular diseases
4.3 Therapeutic targeting of GPCRs in neurological disorders
4.4 GPCR signaling in immune responses
4.5 GPCR signaling in metabolic diseases
4.6 Future directions in GPCR research

Chapter 5: Conclusion and Summary
5.1 Summary of key findings
5.2 Implications of GPCR research
5.3 Future perspectives

Thesis Overview on Cellular and Molecular Pharmacology of G-Protein Coupled Receptors

The study of GPCRs is a rapidly evolving field that has significant implications for drug development and therapy. This thesis will provide a comprehensive overview of the cellular and molecular pharmacology of GPCRs, with a focus on the mechanisms by which these receptors transduce extracellular signals into intracellular responses. The research methodology chapter will detail the experimental techniques used to study GPCRs, including cell culture, transfection, and functional assays. The discussion of findings chapter will explore the role of GPCRs in various diseases and the potential for therapeutic targeting of these receptors. The conclusion and summary chapter will highlight the key findings of the study and discuss future directions in GPCR research.

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