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Table of Content:

Chapter 1: Introduction
1.1 Background of the Study
1.2 Research Problem
1.3 Research Questions
1.4 Research Objectives
1.5 Significance of the Study
1.6 Organization of the Thesis

Chapter 2: Literature Review
2.1 Introduction to Terpenoids
2.2 Biosynthesis of Terpenoids in Plants
2.3 Plant Hairy Root Cultures
2.4 Previous Studies on Biosynthesis of Novel Terpenoids
2.5 Gaps in Literature

Chapter 3: Research Methodology
3.1 Research Design
3.2 Selection of Plant Species and Hairy Root Culture Establishment
3.3 Extraction and Analysis of Terpenoids
3.4 Data Collection and Analysis
3.5 Ethical Considerations

Chapter 4: Discussion of Findings
4.1 Analysis of Terpenoid Profiles in Plant Hairy Root Cultures
4.2 Identification of Novel Terpenoids
4.3 Comparison with Previous Studies
4.4 Implications of Findings

Chapter 5: Conclusion and Summary
5.1 Summary of Findings
5.2 Conclusion
5.3 Recommendations for Future Research

Brief Overview on Biosynthesis of novel terpenoids using plant hairy root cultures:

Terpenoids are a diverse class of natural compounds with various biological activities, including antimicrobial, anti-inflammatory, and anticancer properties. They are commonly found in plants and are synthesized through the mevalonate pathway. Plant hairy root cultures have emerged as a promising tool for the production of terpenoids due to their ability to mimic the biosynthetic pathways present in intact plants.

In this study, we aim to explore the biosynthesis of novel terpenoids using plant hairy root cultures. The research will involve the selection of a suitable plant species and the establishment of hairy root cultures through Agrobacterium rhizogenes-mediated transformation. The terpenoid profiles of the hairy root cultures will be analyzed using techniques such as gas chromatography-mass spectrometry to identify any novel compounds.

Through this research, we hope to contribute to the understanding of terpenoid biosynthesis and potentially discover new bioactive compounds with pharmaceutical or industrial applications. The findings of this study may have implications for the development of novel therapeutics or natural products derived from plant sources.

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