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Introduction

DNA methylation is a key epigenetic modification that plays a critical role in gene regulation and cellular differentiation. It involves the addition of a methyl group to the 5′ position of cytosine residues in the dinucleotide sequence CpG. The pattern of DNA methylation is essential for normal development and cellular function, and aberrant DNA methylation patterns have been associated with various diseases, including cancer.

This thesis aims to explore the biochemistry of DNA methylation, focusing on the mechanisms underlying this epigenetic modification and its implications for gene expression and cellular function. By gaining a better understanding of the biochemistry of DNA methylation, we can potentially develop new therapeutic strategies for diseases associated with aberrant DNA methylation patterns.

Chapter 1: Biochemistry of DNA Methylation

1.1 Introduction
1.2 Background of study
1.3 Problem Statement
1.4 Objective of study
1.5 Limitation of study
1.6 Scope of study
1.7 Significance of study
1.8 Structure of the Thesis
1.9 Definition of terms

Chapter 2: Literature Review

2.1 Overview of DNA methylation
2.2 DNA methyltransferases
2.3 Regulation of DNA methylation
2.4 DNA demethylation mechanisms
2.5 Role of DNA methylation in gene regulation
2.6 DNA methylation in development and differentiation
2.7 DNA methylation in disease
2.8 DNA methylation as a therapeutic target
2.9 Techniques for studying DNA methylation
2.10 Future directions in DNA methylation research

Chapter 3: Research Methodology

3.1 Research design
3.2 Sample collection and preparation
3.3 DNA extraction and bisulfite conversion
3.4 DNA methylation analysis
3.5 Statistical analysis
3.6 Validation of results
3.7 Ethical considerations
3.8 Data management

Chapter 4: Discussion of Findings

4.1 Analysis of DNA methylation patterns
4.2 Identification of differentially methylated regions
4.3 Comparison with existing literature
4.4 Implications for gene expression
4.5 Potential therapeutic implications
4.6 Limitations of the study
4.7 Future directions
4.8 Conclusion

Chapter 5: Conclusion and Summary

In conclusion, this thesis provides a comprehensive overview of the biochemistry of DNA methylation, highlighting the importance of this epigenetic modification in gene regulation and cellular function. By understanding the mechanisms underlying DNA methylation, we can potentially develop new therapeutic strategies for diseases associated with aberrant DNA methylation patterns. The findings of this study contribute to the growing body of knowledge on DNA methylation and its implications for human health and disease.

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