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Introduction

Application of environmental DNA (eDNA) techniques has emerged as a powerful tool in monitoring and tracking the distributions of protected species. eDNA refers to the genetic material shed by organisms into their surrounding environment. This genetic material can be extracted from various environmental samples such as water, soil, and air, providing a non-invasive and cost-effective method for detecting the presence of species of interest.

Protected species are those designated by governments or conservation organizations for special legal protection due to their vulnerable status. Monitoring the distributions of these species is crucial for conservation efforts, as it allows for the assessment of population trends, habitat preferences, and potential threats. Traditional monitoring methods for protected species, such as visual surveys and trapping, can be labor-intensive, time-consuming, and disruptive to the animals and their habitats. eDNA techniques offer a more efficient and less invasive alternative for monitoring these species.

This thesis aims to explore the application of eDNA techniques in monitoring protected species distributions. It will review the current literature on eDNA techniques, discuss the methodology for using these techniques in the field, present findings from case studies, and provide recommendations for future research and conservation efforts.

Table of Contents

Chapter 1: Introduction
1.1 Introduction
1.2 Background of Study
1.3 Problem Statement
1.4 Objective of Study
1.5 Limitation of Study
1.6 Scope of Study
1.7 Significance of Study
1.8 Structure of the Thesis
1.9 Definition of Terms

Chapter 2: Literature Review
2.1 Overview of eDNA Techniques
2.2 Applications of eDNA in Conservation Biology
2.3 eDNA for Monitoring Protected Species
2.4 Comparison of eDNA Techniques with Traditional Methods
2.5 Challenges and Limitations of eDNA Technology
2.6 Case Studies of eDNA Monitoring for Protected Species
2.7 Advances and Innovations in eDNA Technology
2.8 Regulations and Ethics of eDNA Sampling
2.9 Future Directions in eDNA Research
2.10 Summary of Literature Review

Chapter 3: Research Methodology
3.1 Study Design
3.2 Sampling Techniques
3.3 eDNA Extraction and Analysis
3.4 Data Interpretation
3.5 Statistical Analysis
3.6 Quality Control Measures
3.7 Fieldwork Protocol
3.8 Ethical Considerations

Chapter 4: Discussion of Findings
4.1 Overview of Study Sites
4.2 Detection of Protected Species Using eDNA
4.3 Comparison with Traditional Monitoring Methods
4.4 Interpretation of Results
4.5 Implications for Conservation Management
4.6 Recommendations for Future Research
4.7 Limitations of the Study
4.8 Strengths and Weaknesses of eDNA Techniques

Chapter 5: Conclusion and Summary
5.1 Summary of Findings
5.2 Conclusions
5.3 Contribution to the Field
5.4 Recommendations for Conservation Practitioners
5.5 Future Research Directions
5.6 Closing Remarks

Thesis Overview

The application of environmental DNA (eDNA) techniques for monitoring protected species distributions is a growing field in conservation biology. This thesis aims to provide a comprehensive review of the use of eDNA techniques for monitoring protected species, including an overview of the current literature, a discussion of research methodology, and an analysis of findings from case studies. By exploring the potential of eDNA technology to improve conservation efforts, this thesis contributes to the development of more efficient and effective monitoring strategies for protected species.

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