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Introduction

Protein methylation is a post-translational modification that plays a crucial role in various cellular processes, including gene expression regulation, signal transduction, and protein-protein interactions. The addition of a methyl group to specific amino acid residues on proteins is catalyzed by various methyltransferase enzymes. The biochemical mechanisms underlying protein methylation are complex and not fully understood. This thesis aims to explore and elucidate the biochemical mechanisms of protein methylation, focusing on the enzymes involved, the substrates targeted, and the functional consequences of protein methylation.

1.1 Introduction
1.2 Background of the study
1.3 Problem statement
1.4 Objective of the study
1.5 Limitation of the study
1.6 Scope of the study
1.7 Significance of the study
1.8 Structure of the thesis
1.9 Definition of terms

Chapter Two: Literature Review
2.1 Overview of protein methylation
2.2 Enzymes involved in protein methylation
2.3 Substrates targeted for protein methylation
2.4 Functional consequences of protein methylation
2.5 Role of protein methylation in gene expression regulation
2.6 Protein methylation in signal transduction pathways
2.7 Protein methylation in protein-protein interactions
2.8 Regulation of protein methylation
2.9 Dysregulation of protein methylation in diseases
2.10 Current research trends in protein methylation

Chapter Three: Research Methodology
3.1 Selection of experimental models
3.2 Isolation and purification of methyltransferase enzymes
3.3 Identification of methylated protein substrates
3.4 Analysis of protein methylation sites
3.5 Functional assays to explore the consequences of protein methylation
3.6 High-throughput approaches to study protein methylation
3.7 Bioinformatics tools for predicting protein methylation sites
3.8 Validation of protein methylation events

Chapter Four: Discussion of Findings
4.1 Characterization of methyltransferase enzymes
4.2 Identification of novel protein substrates for methylation
4.3 Mapping of protein methylation sites
4.4 Functional consequences of protein methylation
4.5 Crosstalk between protein methylation and other post-translational modifications
4.6 Implications of protein methylation in disease pathogenesis
4.7 Comparison with other post-translational modifications
4.8 Future directions in protein methylation research

Chapter Five: Conclusion and Summary
5.1 Summary of key findings
5.2 Implications of the study
5.3 Future research directions
5.4 Conclusion

Thesis Overview

Protein methylation is a prevalent post-translational modification that plays essential roles in various cellular processes. This thesis aims to explore the biochemical mechanisms of protein methylation, focusing on the enzymes involved, the substrates targeted, and the functional consequences of protein methylation. The literature review will provide an overview of protein methylation, the enzymes involved, the substrates targeted, and the functional consequences, while the research methodology will outline the experimental approaches used to study protein methylation. The discussion of findings will present the characterization of methyltransferase enzymes, the identification of protein substrates for methylation, and the functional consequences of protein methylation. The conclusion will summarize the key findings, discuss the implications of the study, propose future research directions, and provide a conclusion on the biochemical mechanisms of protein methylation.

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