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Introduction

Marine environmental DNA (eDNA) is a rapidly evolving field that has revolutionized our ability to monitor and study marine ecosystems. By extracting and analyzing DNA from environmental samples such as water or sediment, scientists can identify the presence of marine organisms without the need for traditional survey methods. This non-invasive approach has the potential to drastically improve our understanding of marine biodiversity and ecosystem dynamics. However, the preservation of eDNA samples is crucial to ensuring accurate and reliable results.

This thesis aims to review and evaluate the current methods of preserving eDNA samples in marine environments. By examining the various techniques available, we can better understand the challenges and limitations that researchers face in this field. Furthermore, the research conducted in this thesis will provide insights into the best practices for eDNA preservation in marine environments, ultimately contributing to the advancement of this innovative field.

Chapter 1: Introduction
1.1 Introduction
1.2 Background of study
1.3 Problem Statement
1.4 Objective of study
1.5 Limitation of study
1.6 Scope of study
1.7 Significance of study
1.8 Structure of the Thesis
1.9 Definition of terms

Chapter 2: Literature Review
2.1 Overview of Marine eDNA
2.2 Importance of eDNA Preservation
2.3 Current Methods of eDNA Preservation
2.4 Effects of Preservation Methods on eDNA Integrity
2.5 Factors Affecting eDNA Stability
2.6 Challenges in eDNA Preservation
2.7 Comparison of Preservation Techniques
2.8 Emerging Technologies in eDNA Preservation
2.9 Case Studies in eDNA Preservation
2.10 Future Directions in eDNA Preservation Research

Chapter 3: Research Methodology
3.1 Research Design
3.2 Sampling Strategy
3.3 Sample Collection and Preservation
3.4 DNA Extraction Methods
3.5 Quantification and Quality Control of eDNA
3.6 Data Analysis Techniques
3.7 Validation of Preservation Methods
3.8 Ethical Considerations

Chapter 4: Discussion of Findings
4.1 Analysis of Preservation Techniques
4.2 Comparison of Preservation Methods
4.3 Factors Influencing eDNA Preservation
4.4 Implications for Marine eDNA Research
4.5 Recommendations for Best Practices
4.6 Future Research Directions

Chapter 5: Conclusion and Summary
5.1 Recap of Key Findings
5.2 Contributions to the Field
5.3 Implications for Marine Conservation
5.4 Limitations of the Study
5.5 Recommendations for Future Research
5.6 Conclusion

Thesis Overview

Marine environmental DNA (eDNA) preservation is a critical aspect of eDNA research that is often overlooked but essential for ensuring the accuracy and reliability of results. This thesis explores the various methods available for preserving eDNA samples in marine environments and evaluates their effectiveness in maintaining DNA integrity and stability. By conducting a comprehensive literature review, research methodology, and discussion of findings, this thesis aims to provide valuable insights into the best practices for eDNA preservation in marine environments.

In the literature review, the importance of eDNA preservation, current preservation methods, challenges, and emerging technologies are discussed. The research methodology outlines the design, sampling strategy, sample collection, DNA extraction, and data analysis techniques used in evaluating eDNA preservation methods. The discussion of findings analyzes the effects of preservation techniques on eDNA integrity, factors influencing preservation, and implications for marine eDNA research.

Overall, this thesis contributes to the advancement of eDNA research by providing recommendations for best practices in eDNA preservation. The findings of this study will have implications for marine conservation efforts and guide future research in this innovative field. Through a thorough examination of eDNA preservation methods, this thesis aims to enhance our understanding of marine biodiversity and ecosystem dynamics.

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