Engineering of enzymes for improved activity in deep eutectic solvent-based biocatalysis – Complete Phd and Masters Thesis

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**Table of Content**

Chapter 1: Introduction
1.1 Background of the Study
1.2 Research Problem
1.3 Research Objectives
1.4 Research Questions
1.5 Significance of the Study
1.6 Structure of the Thesis

Chapter 2: Literature Review
2.1 Overview of Enzyme Engineering
2.2 Deep Eutectic Solvents (DES) in Biocatalysis
2.3 Enzyme Activity in DES
2.4 Previous Studies on Engineering Enzymes in DES
2.5 Gaps in the Existing Literature

Chapter 3: Research Methodology
3.1 Research Design
3.2 Sample Selection
3.3 Data Collection
3.4 Data Analysis Techniques
3.5 Ethical Considerations

Chapter 4: Discussion of Findings
4.1 Enzyme Engineering Strategies
4.2 Effect of DES on Enzyme Activity
4.3 Comparison of Engineered Enzymes in DES
4.4 Implications of Findings

Chapter 5: Conclusion and Summary
5.1 Summary of Key Findings
5.2 Contributions to the Field
5.3 Practical Implications
5.4 Recommendations for Future Research

**Brief Overview: Engineering of Enzymes for Improved Activity in Deep Eutectic Solvent-Based Biocatalysis**

Enzymes are biological catalysts that play a crucial role in various industrial processes, including biocatalysis. Deep eutectic solvents (DES) have recently emerged as promising media for enzyme-catalyzed reactions due to their unique properties such as low toxicity, biodegradability, and ability to stabilize enzymes.

However, the activity of enzymes in DES is often suboptimal, leading to the need for enzyme engineering to enhance their performance. This project aims to investigate different strategies for engineering enzymes to improve their activity in DES-based biocatalysis.

The literature review will examine previous studies on enzyme engineering in DES, identifying gaps in the existing literature and laying the foundation for the research methodology. The research will focus on selecting appropriate enzymes, designing and conducting experiments in DES, analyzing the data, and drawing conclusions based on the findings.

The discussion of findings will evaluate the effectiveness of enzyme engineering strategies in enhancing enzyme activity in DES and provide recommendations for future research in this area. The conclusion will summarize the key findings and their implications for the field of biocatalysis.

Overall, this project will contribute to the advancement of enzyme engineering in DES-based biocatalysis, offering new insights into optimizing enzyme performance for industrial applications.

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