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Table of Contents:

Chapter 1: Introduction
– Background of the study
– Significance of the study
– Research aim and objectives
– Research questions
– Structure of the thesis

Chapter 2: Literature Review
– Overview of shikimic acid
– Metabolic engineering concepts
– Previous studies on metabolic engineering of yeast for shikimic acid production
– Challenges and opportunities in metabolic engineering of yeast for shikimic acid production

Chapter 3: Research Methodology
– Research design
– Yeast strain selection
– Genetic modification techniques
– Fermentation process optimization
– Analytical methods for shikimic acid quantification

Chapter 4: Discussion of Findings
– Characterization of the engineered yeast strain
– Shikimic acid production kinetics
– Optimization of fermentation parameters
– Comparison with previous studies
– Implications of the findings

Chapter 5: Conclusion and Summary
– Summary of key findings
– Interpretation of results
– Contributions to the field of metabolic engineering
– Recommendations for future research
– Conclusion

Brief Overview:

Metabolic engineering is a powerful tool that allows researchers to modify the metabolism of microorganisms for the production of valuable compounds. Shikimic acid is an important building block for the synthesis of pharmaceuticals, including the anti-influenza drug oseltamivir (Tamiflu). Currently, shikimic acid is mainly produced from the Chinese star anise plant, which has limited availability and high production costs.

This project focuses on the metabolic engineering of yeast for the production of shikimic acid as an alternative, cost-effective production method. The research will involve selecting a suitable yeast strain, introducing genes involved in the shikimic acid biosynthetic pathway, optimizing fermentation conditions, and quantifying shikimic acid production.

The literature review will provide a comprehensive overview of shikimic acid production, metabolic engineering principles, and previous studies on yeast metabolic engineering for shikimic acid production. The research methodology will detail the experimental design, genetic modification techniques, fermentation optimization, and analytical methods used in the study.

The discussion of findings will include the characterization of the engineered yeast strain, shikimic acid production kinetics, fermentation parameter optimization, comparison with previous studies, and implications of the findings. The conclusion and summary will summarize the key findings, interpret the results, discuss contributions to the field, provide recommendations for future research, and present a conclusion.

Overall, this project aims to demonstrate the feasibility of using metabolic engineering to produce shikimic acid in yeast and contribute to the development of sustainable and cost-effective production processes for valuable compounds in the pharmaceutical industry.

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