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INTRODUCTION

Small RNAs are a class of non-coding RNAs that play a crucial role in gene regulation. They are typically around 20-30 nucleotides in length and are involved in a variety of cellular processes, including transcriptional and post-transcriptional gene silencing. Small RNAs can regulate gene expression by targeting mRNAs for degradation or by inhibiting their translation.

BACKGROUND OF STUDY

Small RNAs have been extensively studied in recent years and have been shown to play a key role in a wide range of biological processes, including development, differentiation, and disease. Understanding the mechanisms by which small RNAs regulate gene expression is critical for unraveling the complexity of gene regulation.

PROBLEM STATEMENT

Despite the significant progress that has been made in understanding the role of small RNAs in gene regulation, many questions still remain unanswered. For example, the precise mechanisms by which small RNAs target specific mRNAs and the factors that influence their activity are still not fully understood.

OBJECTIVE OF STUDY

The main objective of this thesis is to provide a comprehensive overview of the role of small RNAs in gene regulation. Specifically, we aim to elucidate the molecular mechanisms by which small RNAs regulate gene expression and to examine the implications of small RNA-mediated gene regulation in health and disease.

LIMITATION OF STUDY

One limitation of this study is that the field of small RNA research is rapidly evolving, with new discoveries being made on a regular basis. As such, some of the information presented in this thesis may become outdated as new research is published.

SCOPE OF STUDY

This thesis will focus on the role of small RNAs, such as microRNAs and siRNAs, in gene regulation in eukaryotic cells. We will examine the various mechanisms by which small RNAs regulate gene expression and discuss their implications for cellular function.

SIGNIFICANCE OF STUDY

Understanding the role of small RNAs in gene regulation has significant implications for human health and disease. Small RNAs have been implicated in a wide range of diseases, including cancer and neurological disorders, and targeting small RNAs for therapeutic purposes is an area of active research.

STRUCTURE OF THE THESIS

Chapter 1: Introduction
1.1 Introduction
1.2 Background of study
1.3 Problem Statement
1.4 Objective of study
1.5 Limitation of study
1.6 Scope of study
1.7 Significance of study
1.8 Structure of the Thesis
1.9 Definition of terms

Chapter 2: Literature Review
2.1 Overview of small RNAs
2.2 Mechanisms of small RNA-mediated gene regulation
2.3 Role of small RNAs in development
2.4 Small RNAs in disease
2.5 Small RNAs as therapeutic targets
2.6 Regulation of small RNA expression
2.7 Small RNAs and epigenetics
2.8 Small RNAs and environmental factors
2.9 Small RNAs and evolution
2.10 Small RNAs in non-eukaryotic organisms

Chapter 3: Research Methodology
3.1 Research design
3.2 Data collection methods
3.3 Data analysis techniques
3.4 Experimental approaches
3.5 Cell culture techniques
3.6 RNA isolation and quantification
3.7 High-throughput sequencing methods
3.8 Bioinformatic analysis tools

Chapter 4: Discussion of Findings
4.1 Mechanisms of small RNA-mediated gene regulation
4.2 Implications for human health and disease
4.3 Therapeutic potential of small RNAs
4.4 Challenges and future directions

Chapter 5: Conclusion and Summary
5.1 Summary of key findings
5.2 Conclusions
5.3 Future directions for research

THESIS OVERVIEW

Small RNAs are a class of non-coding RNAs that play a crucial role in gene regulation. This thesis provides a comprehensive overview of the role of small RNAs in gene regulation, focusing on mechanisms of small RNA-mediated gene regulation, implications for human health and disease, and therapeutic potential. The study aims to elucidate the molecular mechanisms by which small RNAs regulate gene expression and to explore the implications of small RNA-mediated gene regulation in health and disease. The thesis consists of five chapters, including an introduction, literature review, research methodology, discussion of findings, and conclusion.

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