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Introduction

Nanocarriers have emerged as promising vehicles for delivering drugs and genes across biological barriers, offering targeted and controlled release, enhanced stability, and improved therapeutic efficacy. This thesis aims to explore the potential of nanocarriers for drug and gene delivery across biological barriers, focusing on their mechanisms of action, design considerations, and applications in various disease contexts.

1.1 Introduction
1.2 Background of study
1.3 Problem Statement
1.4 Objective of study
1.5 Limitation of study
1.6 Scope of study
1.7 Significance of study
1.8 Structure of the Thesis
1.9 Definition of terms

Chapter Two: Literature Review
2.1 Nanocarriers for drug delivery
2.2 Nanocarriers for gene delivery
2.3 Biological barriers in drug and gene delivery
2.4 Design considerations for nanocarriers
2.5 Applications of nanocarriers in disease therapy
2.6 Challenges and opportunities in nanocarrier technology
2.7 Recent advancements in nanocarrier research
2.8 Clinical translation of nanocarriers
2.9 Regulatory considerations for nanocarrier-based therapies
2.10 Future perspectives in nanocarrier research

Chapter Three: Research Methodology
3.1 Selection of nanocarrier systems
3.2 Loading and release kinetics studies
3.3 In vitro and in vivo models for biological barrier evaluation
3.4 Characterization of nanocarrier formulations
3.5 Assessment of drug and gene delivery efficiency
3.6 Safety and toxicity evaluation
3.7 Statistical analysis of data
3.8 Ethical considerations in research

Chapter Four: Discussion of Findings
4.1 Nanocarrier design and performance
4.2 Mechanisms of drug and gene delivery across biological barriers
4.3 Comparison of different nanocarrier systems
4.4 Optimization strategies for enhanced delivery efficiency
4.5 Safety and regulatory considerations
4.6 Challenges and future directions in nanocarrier research

Chapter Five: Conclusion and Summary
5.1 Summary of key findings
5.2 Contributions to the field of nanocarrier research
5.3 Implications for future research and clinical applications
5.4 Concluding remarks

Thesis Overview

Nanocarriers hold great promise in revolutionizing drug and gene delivery across biological barriers, offering targeted and efficient therapeutic interventions. This thesis aims to provide a comprehensive overview of the current state of nanocarrier technology, from design considerations to clinical translation, with a specific focus on addressing biological barriers in drug and gene delivery. By reviewing existing literature, conducting experimental research, and analyzing the findings, this thesis seeks to advance our understanding of nanocarrier-based therapies and their potential impact on disease treatment. The structured approach of this thesis, with chapters dedicated to literature review, research methodology, discussion of findings, and conclusion, will provide a coherent framework for assessing the efficacy and safety of nanocarriers for drug and gene delivery. Ultimately, this thesis aims to contribute to the growing body of knowledge in the field of nanomedicine and inspire further research in developing innovative nanocarrier technologies for clinical applications.

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