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Introduction

Genetic disorders have long been a challenge in the field of medicine, with limited treatment options available. Angelman syndrome is a rare neurogenetic disorder characterized by intellectual disability, developmental delays, seizures, and speech impairments. Currently, there is no cure for Angelman syndrome, and treatment options are focused on managing symptoms rather than addressing the underlying cause of the disorder.

Recent advancements in gene editing technologies, particularly CRISPR-Cas9, have shown promise in potentially treating genetic disorders by targeting and modifying specific genes. CRISPR-based gene editing offers a precise and efficient way to correct genetic mutations that cause diseases, including Angelman syndrome.

This thesis aims to investigate the potential of CRISPR-based gene editing for the treatment of Angelman syndrome. By exploring the current research and developments in this field, this study aims to provide insights into the feasibility and challenges of using CRISPR technology to address the genetic cause of Angelman syndrome.

1.1 Introduction
1.2 Background of study
1.3 Problem Statement
1.4 Objective of study
1.5 Limitation of study
1.6 Scope of study
1.7 Significance of study
1.8 Structure of the Thesis
1.9 Definition of terms

Chapter 2: Literature Review
2.1 Overview of Angelman syndrome
2.2 Genetics of Angelman syndrome
2.3 Current treatment options for Angelman syndrome
2.4 CRISPR-Cas9 technology
2.5 Applications of CRISPR in genetic disorders
2.6 Studies on CRISPR-based gene editing for Angelman syndrome
2.7 Challenges and limitations of CRISPR technology
2.8 Ethical considerations in gene editing
2.9 Future prospects of CRISPR gene editing for Angelman syndrome
2.10 Gaps in current research on CRISPR for Angelman syndrome

Chapter 3: Research Methodology
3.1 Research design
3.2 Sample selection
3.3 Data collection methods
3.4 Data analysis
3.5 Ethical considerations
3.6 Budget and timeline
3.7 Collaboration and resources
3.8 Potential challenges and their mitigation

Chapter 4: Discussion of Findings
4.1 Summary of research findings
4.2 Analysis of results
4.3 Comparison with existing literature
4.4 Implications for the treatment of Angelman syndrome
4.5 Future research directions
4.6 Limitations of the study
4.7 Recommendations for clinical practice
4.8 Policy implications

Chapter 5: Conclusion and Summary
5.1 Summary of key findings
5.2 Contributions to the field
5.3 Implications for future research
5.4 Practical applications of the study
5.5 Conclusion and final remarks

Thesis Overview

The goal of this thesis is to explore the potential of CRISPR-based gene editing for the treatment of Angelman syndrome. The introduction provides background information on Angelman syndrome, the limitations of current treatment options, and the rationale for using CRISPR technology to address the genetic cause of the disorder.

The literature review examines the genetics of Angelman syndrome, current treatment options, and the potential applications of CRISPR technology in treating genetic disorders. The chapter also discusses the challenges, ethical considerations, and future prospects of using CRISPR for Angelman syndrome.

The research methodology chapter outlines the design, sample selection, data collection methods, and ethical considerations of the study. It also addresses budget, timeline, collaboration, and potential challenges that may arise during the research process.

The discussion of findings chapter presents a summary of research findings, analysis of results, implications for the treatment of Angelman syndrome, and recommendations for future research and clinical practice. The chapter also highlights the limitations of the study and policy implications of using CRISPR for genetic disorders.

In the conclusion and summary chapter, key findings are summarized, contributions to the field are discussed, implications for future research are outlined, and practical applications of the study are presented. The chapter concludes with final remarks on the potential of CRISPR-based gene editing for the treatment of Angelman syndrome.

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