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Introduction

In recent years, microfluidic devices have gained significant attention in the field of biosensing applications due to their potential to provide high sensitivity, rapid analysis, and low sample consumption. These devices offer a platform for the manipulation and control of small volumes of fluids at the microscale level, leading to the development of portable, cost-effective, and highly efficient biosensors for various applications such as healthcare, environmental monitoring, and food safety.

This thesis focuses on the design and development of a microfluidic device for biosensing applications. The device aims to detect and quantify specific biological targets, such as proteins, nucleic acids, and cells, in complex samples with high precision and sensitivity. The integration of microfluidics with biosensing technologies has the potential to revolutionize the field of diagnostics by providing point-of-care testing, real-time monitoring, and personalized medicine.

Chapter 1: Introduction
1.1 Introduction
1.2 Background of Study
1.3 Problem Statement
1.4 Objective of Study
1.5 Limitation of Study
1.6 Scope of Study
1.7 Significance of Study
1.8 Structure of Thesis
1.9 Definition of Terms

Chapter 2: Literature Review
2.1 Overview of Microfluidic Devices
2.2 Biosensing Technologies
2.3 Integration of Microfluidics and Biosensing
2.4 Applications of Microfluidic Biosensors
2.5 Design Considerations for Microfluidic Devices
2.6 Fabrication Techniques for Microfluidic Devices
2.7 Recent Advancements in Microfluidic Biosensors
2.8 Challenges and Limitations in Microfluidic Biosensing
2.9 Future Perspectives in Microfluidic Biosensing
2.10 Summary of Literature Review

Chapter 3: Research Methodology
3.1 Research Design
3.2 Selection of Materials
3.3 Design and Fabrication of Microfluidic Device
3.4 Functionalization of Biosensors
3.5 Testing and Validation
3.6 Data Analysis
3.7 Performance Evaluation
3.8 Ethical Considerations

Chapter 4: Discussion of Findings
4.1 Performance of Microfluidic Device
4.2 Sensitivity and Specificity of Biosensors
4.3 Comparison with Conventional Methods
4.4 Optimization Strategies
4.5 Influence of Operating Parameters
4.6 Implications for Biosensing Applications
4.7 Future Directions
4.8 Conclusion of Findings

Chapter 5: Conclusion and Summary
5.1 Summary of Findings
5.2 Contributions to the Field
5.3 Practical Implications
5.4 Recommendations for Future Research
5.5 Conclusion

Overall, this thesis aims to contribute to the advancement of microfluidic biosensing technology by designing and developing a novel device for biosensing applications. The integration of microfluidics and biosensing holds great potential for improving healthcare diagnostics, environmental monitoring, and food safety, leading to more efficient and reliable detection methods.

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