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Introduction

Cancer is a complex disease that arises from the dysregulation of cell cycle progression, leading to uncontrolled cell proliferation. The role of microRNAs (miRNAs) in cancer has been widely studied, as they play a crucial role in the regulation of gene expression at the post-transcriptional level. In recent years, there has been increasing evidence suggesting that miRNAs are involved in the control of cell cycle progression in cancer cells. Understanding the mechanisms by which miRNAs regulate the cell cycle in cancer cells could provide valuable insights into the development of novel therapeutic strategies for cancer treatment.

Chapter 1: Introduction
1.1 Introduction
1.2 Background of the study
1.3 Problem statement
1.4 Objective of the study
1.5 Limitation of the study
1.6 Scope of the study
1.7 Significance of the study
1.8 Structure of the thesis
1.9 Definition of terms

Chapter 2: Literature review
2.1 Overview of cancer cell cycle regulation
2.2 Role of miRNAs in cancer
2.3 miRNAs involved in cell cycle regulation
2.4 Dysregulation of miRNAs in cancer cells
2.5 Therapeutic potential of targeting miRNAs in cancer
2.6 Methods for miRNA detection and analysis
2.7 miRNA target prediction algorithms
2.8 Experimental approaches to study miRNA function
2.9 Challenges in miRNA research
2.10 Future directions in miRNA-based cancer therapy

Chapter 3: Research methodology
3.1 Research design
3.2 Study population
3.3 Data collection methods
3.4 miRNA profiling techniques
3.5 Cell culture and transfection
3.6 Cell cycle analysis
3.7 Gene expression analysis
3.8 Statistical analysis

Chapter 4: Discussion of findings
4.1 miRNAs involved in cell cycle regulation in cancer cells
4.2 Dysregulated miRNAs in cancer cells
4.3 Functional analysis of candidate miRNAs
4.4 Regulatory mechanisms of miRNAs in the cell cycle
4.5 Implications for cancer therapy
4.6 Comparison with existing literature
4.7 Limitations of the study
4.8 Future research directions

Chapter 5: Conclusion
5.1 Summary of findings
5.2 Implications of the study
5.3 Recommendations for future research
5.4 Conclusion

Thesis Overview:
Investigating the role of microRNAs in the regulation of cell cycle progression in cancer cells is a comprehensive study that aims to elucidate the mechanisms by which miRNAs control cell cycle progression in cancer cells. The thesis is divided into five chapters, starting with an introduction that provides background information on the study, outlines the research objectives, and discusses the significance of the research. The literature review chapter presents a detailed analysis of the current understanding of miRNAs in cancer cell cycle regulation. The research methodology chapter describes the experimental approaches used to investigate the role of miRNAs in cancer cell cycle regulation, including miRNA profiling, cell culture, and gene expression analysis. The discussion of findings chapter presents the results of the study, including the identification of dysregulated miRNAs and their functional analysis in cancer cells. Finally, the conclusion chapter summarizes the key findings of the study, discusses their implications, and outlines recommendations for future research in this field.

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