Developing a protocol for the extraction and analysis of DNA from charred remains – Complete Phd and Masters Thesis

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Thesis Overview:

Title: Developing a Protocol for the Extraction and Analysis of DNA from Charred Remains

Introduction:
DNA analysis has revolutionized forensic science by providing crucial evidence in criminal investigations, identifying missing persons, and establishing genetic relationships. However, the extraction and analysis of DNA from charred remains present unique challenges due to the degradation of DNA caused by high temperatures. This thesis aims to develop a protocol for the extraction and analysis of DNA from charred remains to enhance the accuracy and reliability of forensic investigations.

Chapter One: Introduction
1.1 Introduction
1.2 Background of Study
1.3 Problem Statement
1.4 Objective of Study
1.5 Limitation of Study
1.6 Scope of Study
1.7 Significance of Study
1.8 Structure of the Thesis
1.9 Definition of Terms

Chapter Two: Literature Review
– Historical perspective of DNA analysis from charred remains
– Challenges and limitations in DNA extraction from charred remains
– Current methods and protocols for DNA extraction from charred remains
– Advances in DNA analysis technology for degraded samples
– Case studies on successful DNA extraction from charred remains

Chapter Three: Research Methodology
– Selection of charred remains samples
– DNA extraction techniques
– DNA quantification and purity assessment
– Polymerase chain reaction (PCR) amplification
– Short Tandem Repeat (STR) analysis
– Mitochondrial DNA analysis
– Quality control measures
– Data analysis

Chapter Four: Discussion of Findings
– Comparison of different DNA extraction methods
– Evaluation of DNA quality and quantity from charred remains
– Optimizing PCR conditions for degraded DNA
– Interpretation of DNA profiles from charred remains
– Implications for forensic investigations

Chapter Five: Conclusion and Summary
– Summary of research findings
– Recommendations for future research
– Practical applications of the developed protocol
– Contribution to the field of forensic science

In conclusion, this thesis will provide valuable insights into the development of a standardized protocol for the extraction and analysis of DNA from charred remains. By addressing the challenges associated with degraded DNA samples, this research aims to enhance the efficiency and accuracy of forensic investigations involving charred remains.

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